The possibilities of applying marker assisted selection in breeding boron tolerant wheat
2013
The aim of this study was to investigate the possibilities of applying
molecular markers-microsatellites in breeding boron tolerant wheat. The study
comprised the investigation of allelic variability of sixty bread wheat
accessions in two microsatellite loci (Xgwm46-7B and Xgwm577-7B) for which
was assumed that are placed near the 7B chromosome locus involved in the
expression of boron tolerance in wheat. Phenotypic variability concerning
boron tolerance was assessed via root length reduction of wheat seedlings
grown in the presence of high external boron, applied as boric acid solution
(concentrations 50, 100 and 150 mg/l, boron treatments B50, B100 and B150).
The indication of marker-trait associations was determined by comparing the
allelic variability in the two microsatellite loci with the phenotypic
variability in boron tolerance. Nonparametric Kruskal-Wallis test was used
for the comparisons. The indication of marker-trait association was found for
both Xgwm46-7B and Xgwm577-7B; on B150 and B50 treatments, respectively.
Allelic forms identified in Xgwm577-7B locus may be related to tolerance,
medium tolerance and sensitivity to high boron. This was not the case for
Xgwm46-7B, where the identified alleles were related only to boron tolerance
and sensitivity. Therefore, Xgwm577-7B may be preferred over Xgwm46-7B when
studying boron tolerance in wheat. However, a considerable portion of boron
tolerant accessions carried different alleles in the investigated loci,
implying boron tolerance as a quantitative trait with more than one
chromosomal region involved in its expression. Therefore, the allelic
variability of more than the analyzed two loci should be investigated.
[Projekat Ministarstva nauke Republike Srbije, br. TR 31066]
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