Study the protective effect and mechanism of combining breviscapine and preconditioning against ischemic reperfusion injury on ischemic reperfusion model of rabbits

Objective:To study the protective effect and mechanism of combining breviscapine(Bre) and preconditioning against ischemic reperfusion(IR) injury on IR model of rabbits.Method:Forty-eight New Zealand rabbits were randomly divided into six groups: sham operation group,IR group,ischemic preconditioning(IPC) group,three different groups of combining Bre(10 mg/kg,5 mg/kg or 1 mg/kg): HBre group,MBre group,LBre group.Each group involved eight rabbits.Heart functional parameters(LVSP,LVEDP and ±dp/dtmax) were recorded before ischemia,40min after ligation and 180 min after repefusion.Concentration of CK and CK-MB in plasma at the three different time point was measured.Heart tissue homogenate(10%) was made and then SOD,MDA and NO were measured after centrifugalization.Result:IPC group and three different doses of Bre group compared with IR group(P0.01),can improve heart functional,and decreased the values of CK,CK-MB,MDA,increase the values of SOD,NO.The HBre group and MBre group were better than IPC group(P0.01).The dose of Bre 10 mg/kg and 5 mg/kg have excellent effects.Conclusion:Combining Bre and preconditioning can strengthen the protective effect of ischemic preconditioning against myocardial ischemic reperfusion injury.The mechanism of protection may relate to significantly eliminate the oxygen free radical,hold back the lipid peroxidation and increase the content of NO.