Effect of infection with a mesogenic strain of Newcastle Disease Virus on infection with Highly Pathogenic Avian Influenza Virus in chickens

SUMMARY. Little is known on the interactions between avian influenza virus (AIV) and Newcastle disease virus (NDV) when coinfecting the same poultry host. In a previous study we found that infection of chickens with a mesogenic strain of NDV (mNDV) can reduce highly pathogenic AIV (HPAIV) replication, clinical disease, and mortality. This interaction depended on the titer of the viruses used and the timing of the infections. To further explore the effect of mNDV infectious dose in protecting chickens against HPAIV infection, 2-wk-old birds were inoculated with different doses of mNDV (104, 106, or 107 50% embryo infective dose [EID50]) 3 days before inoculation with a HPAIV (105 or 106 EID50). Although birds coinfected with the higher mNDV doses (106 or 107) survived for longer than birds inoculated only with HPAIV (105), we did not observe the same protection with the lower dose of mNDV (104) or when given the higher dose of HPAIV (106), indicating that the relation between the titer of the two coinfecti...