Co-localization of proteins with defined sequential order and controlled stoichiometric ratio on magnetic nanoparticles

Co-immobilization of enzymes used in cascade reactions is important for improving the overall catalytic efficiency. In this work, we employed scaffoldins as a bridge and succeeded in a highly-ordered co-localization of multiple proteins on magnetic nanoparticles with a loading capacity of ∼0.831 μmol g−1 supports.