Platelet adhesion and activation on chiral surfaces: the influence of protein adsorption

Adsorbed protein and its conformational change on blood-contacting biomaterials will determine the final hemocompatibility. It has been reported frequently that surface chirality of biomaterials may highly influence protein adsorption behavior. Herein, lysine and tartaric acid with different chirality were immobilized onto TiO2 films respectively, and the influence of surface chirality on protein adsorption, platelet adhesion and activation was also investigated. It showed that the L- and D-molecule grafted samples had almost the same grafting density, surface topography, chemical components and hydrophilicity in this study. However, biological behaviors such as protein adsorption, platelet adhesion and activation were quite different. The D-lysine grafted surface had greater ability to inhibit both bovine serum albumin and fibrinogen adsorption, along with less degeneration of fibrinogen than that of L-lysine anchored surface. While the D-tartaric acid grafted surface adsorbed more protein but with less ...