Simple Methods for Production of HL‐A Antigen Poor Red Blood Cells

Six simple methods for the production of HL-A antigen poor blood were compared. By combinations of conventional filtration and centrifugation technics, it was possible to remove more than 95 per cent of platelets and leukocytes, which compares favorably to frozen, washed erythrocyte products. Double centrifugation (upright, then inverted) allowed recovery of all components with excellent removal of leukocytes and platelets and low erythrocyte loss. A special inverted plasma expressor shortened preparation time significantly.