Preliminary study on influence of filtration or centrifugation on pyrogen test

Objective To confirm the influence of filtration or centrifugation on pyrogen test.Methods Kinetic turbidity test was performed to detect bacterial endotoxin concentration in the solution after filtration or centrifugation according to the 2010 Edition of Chinese Pharmacopoeia.The endotoxin concentration in the primary solution(0.2 EU/mL) was detected after filtration and centrifugation,respectively.Results The bacterial endotoxin concentration in the solution were reduced after filtration(less than 0.040 EU/mL remaining),whereas centrifugation didn't influence the endotoxin concentration in the solution(0.260 EU/mL remaining).Conclusion It is demonstrated that centrifugation could be utilized to eliminate particles in the solution for pyrogen test.