Allergen‐induced histamine secretion associated with lactate production in mast cells detected by 1H NMR

Allergen-induced histamine secretion in mast cells, obtained from rats infected with Nippostrongylus brasiliensis, was detected by a 1 H NMR semi-selective excitation technique. Lactate production was also detected in association with histamine secretion as observed in mast cells stimulated with other nonimmunological agents, such as compound 48/80, polymyxin B, and melittin. The assignment of the lactate resonance was confirmed using lactate oxidase. Thus, 1 H NMR spectroscopy is a useful method to study the activation of mast cells in allergic reaction