РАЗРАБОТКА МЕТОДИК КОЛИЧЕСТВЕННОГО ОПРЕДЕЛЕНИЯ СОДЕРЖАНИЯ B-КАРОТИНА И ФИКОЦИАНИНА В БИОМАССЕ СПИРУЛИНЫ ПИЩЕВОЙ (SPIRULINA PLATENSIS)

In the present paper are discussed the results of the investigations in the fi eld of standardization of biomass of Spirulina platensis, cultivated in the Samara region. The methods of quantitative determination of the most important biologically active compounds of Spirulina platensis – β-carotene and phycocyanin was developed. There was developed the method of quantitative determination of β-carotene using chromatospectrophotometry with analytical wavelength at 450 nm. There was established that the content of β-carotene in the Spirulina platensis biomass varies from 20,90 to 40,85 mg %. The relative degree of the determination of the the content of β-carotene in developed method with confi dence probability 0,95 is no more than +4,45 %. There was developed the technique of quantitative defi nition of phycocyanin using spectrophotometry with the analytical wavelength 620 nm. There was established that the content of phycocyanin in the Spirulina platensis biomass varies from 5,57 to 10,05 %. The relative degree of the determination of the content of phycocyanin in developed method with confi dence probability 0,95 is no more than + 4,07 %.