Actions of cyclosporin A on cultured rat mesangial cells

Actions of cyclosporin A on cultured rat mesangial cells. The effects of cyclosporin A (CsA) on planar surface area of cultured rat mesangial cells (PCSA) and cross-sectional area of isolated rat glomeruli (GCSA) were tested. The same experiments were performed after preincubation with platelet activating factor (PAF) antagonists (BN 52021, alprazolam) or calcium channel blockers (verapamil). Areas of cells or glomeruli were analyzed by a computer-assisted method. CsA reduced PCSA in a time-dependent (significant effects began at 15 min, about 12% of reduction with 10 -6 M CsA) and dose-dependent (no effect at 10 -9 M CsA, maximal reduction of about 30% at 60min of incubation with 10 -7 M CsA) fashion. BN 52021 (5.10 -5 M) and alprazolam (10 -5 M) completely inhibited the reduction of mesangial cell area induced by CsA. Verapamil (10 -5 M) only partially inhibited this action. Glomerular cross-sectional area decreased after 30 minutes of incubation with 10 -6 M CsA (1.45 ± 0.02 vs. 1.55 ± 0.02m 2 · 10 -8 , P -6 M CsA increased PAF production by isolated rat glomeruli (425 ± 80 pg/mg vs. 198 ± 13 pg/mg in control glomeruli, P