Imaging of Gene Expression in Live Pancreatic Islet Cell Lines Using Dual-Isotope SPECT

tablish a sensitive, quantitative, and tomographic method with which to detect gene expression in pancreatic islet cells in vivo. Dual-isotope SPECT can be used to image multiple molecular events simultaneously, and coregistration of SPECT and CT images enables visualization of reporter gene expression in the correct anatomic context. We have engineered pancreatic islet cell lines for imaging with SPECT/CT after transplantation under the kidney capsule. Methods: INS-1 832/13 and aTC1-6 cells were stably transfected with a herpes simplex virus type