MOLECULAR FINGERPRINTING METHODS FOR STUDIES INVOLVING ORAL Candida albicans TÍTULO Métodos de caracterização molecular para estudos envolvendo Candida albicans de origem bucal
2009
OBJECTIVE: This review was aimed to discuss the literature concerning the fingerprint methods for epidemiological studies of oral-borne Candida albicans. DISCUSSION: Interest in obtaining a better understanding of the pathogenesis, epidemiology, genetics and evolution of Candida albicans has led to the development of innumerable investigations. These studies have employed fingerprinting systems, such as multilocus enzyme electrophoresis, electrophoretic karyotyping, randomly amplified polymorphic DNA, and restriction length fragment polymorphism, with and without hybridization. The efficacy of these systems has been examined at different levels of discrimination. A validation strategy has been delineated which compares two or more unrelated methods. Moreover, the different fingerprinting patterns produced could be registered in database programs and submitted to comparison with parameters of the host and characteristics of the pathogen. These procedures permit urrent and retrospective comparison of a selection of clinical and epidemiologically important strains, which could show one or several characteristics of the host or pathogen. Additionally, the sum of this growing amount of information could contribute even more to the understanding of the dynamics of infectious organisms in human populations, the complex relationship between commensalism and infection, and genetic and evolutionary mechanisms. CONCLUSIONS: Multiple molecular systems are available for studies involving C. albicans. This growing amount of information contributes to the understanding of the dynamics of this fungus in human populations.
Keywords:
- Correction
- Cite
- Save
- Machine Reading By IdeaReader
196
References
0
Citations
NaN
KQI