Burkholderia zhejiangensis sp. nov., a methyl-parathion-degrading bacterium isolated from a wastewater-treatment system

The taxonomic status of a methyl-parathion-degrading strain, OP-1 T , isolated from a wastewatertreatment system in China, was determined using a polyphasic approach. The rod-shaped cells were Gram-staining-negative, non-spore-forming and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain belonged to the genus Burkholderia ,a s it appeared closely related to Burkholderia glathei ATCC 29195 T (97.4% sequence similarity), Burkholderia sordidicola KCTC 12081 T (96.5%) and Burkholderia bryophila LMG 23644 T (96.3%). The major cellular fatty acids, C16:0 ,C 17:0 cyclo and C18:1v7c, were also similar to those found in established members of the genus Burkholderia. The genomic DNA G+C content of strain OP-1 T was 59.4 mol%. The level of DNA–DNA relatedness between the novel strain and the closest recognized species, Burkholderia glathei ATCC 29195 T , was only 30%. Based on the phenotypic, genotypic and phylogenetic evidence, strain OP-1 T represents a novel species of the genus Burkholderia, for which the name Burkholderia zhejiangensis sp. nov. is proposed. The type strain is OP-1 T (5CCTCC AB 2010354 T 5KCTC 23300 T ).