Monoclonal antibody Ki-67 identifies B and T cells in cycle in chronic lymphocytic leukemia : correlation with disease activity

Ki-67 is a monoclonal antibody that recognises a nuclear antigen expressed during most phases of the cell cycle. We have analysed, by immunocytochemistry, the frequence, morphology, and clinical significance of Ki-67 + cells in 108 patients with B-cell chronic lymphocytic leukemia (CLL). Because in normal peripheral blood Ki-67 + cells are mainly T lymphocytes, we have also investigated, by double immunoenzymatic staining, the proportion of K1-67 + T cells (Ki-67/CD3 + ) in CLL