The relationship between GAPDH mRNA degradation in the mouse liver and postmortem interval
2010
Objective To explore the relationship between degradation of glyceraldehyde-3-phosphate dehydrogenase(GAPDH) mRNA in the mouse liver and postmortem interval(PMI).Methods Sixty NIH mice were sacrificed by cervical dislocation and suffocation,and then placed into 10 ℃ and 25 ℃ temperature-controlling systems.The changes of GAPDH mRNA in the liver were detected by two-step fluorimetric reverse transcriptase polymerase chain reaction(RT-PCR) technique and nucleic acids protein cryoscope from 0 to 48 h postmortem.Results In the mouse liver,the amplification products of GAPDH mRNA could be examined within 48 h postmortem in 10 ℃ temperature-controlling system and within 36 h postmortem in 25 ℃ temperature-controlling system.The amplification products showed a decreasing tendency.Conclusion Degradation of GAPDH mRNA in the mouse liver is negative correlation with PMI.GAPDH mRNA could be a new marker for estimation of PMI.
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