Forensic determination of crossing lines involving stamp and pen inks by mass spectrometry imaging

A most challenge task for the forensic cases is to identify the intersection sequence of lines from inks in questioned documents, mainly in those involving significant financial values such as insurance claims, wills and contracts. The optical examinations by microscope images have been commonly used, but this morphological examination presents severe limitations that can lead to false conclusions. Herein, we show that mass spectrometry imaging (MSI) using easy ambient sonic-spray ionization (EASI) can be used to accurately identify the sequence of lines in intersecting points between stamp and pen inks. First, we used proof of concept cases and ion images, extracted ion chronograms and EASI-MS profiles to evaluate how they could identify the intersection sequences. EASI-MSI was then used to distinguish the sequence of intersecting lines in two cases of simulated contract documents in which a signature from a pen ink was placed under or above the stamp ink. We demonstrate that EASI-MSI provided clear indication for the actual sequence, showing to function as an efficient and rather unequivocal analytical tool in the examination of crossing ink lines when stamping is involved.