A long-term lifetime amperometric glucose sensor with a perfluorocarbon polymer coating

Abstract We have developed an amperometric glucose sensor whose electrodes are coated with a four-layered membrane: 3-aminopropyltriethoxysilane ( γ -APTES), Nafion®, glucose oxidase (GOX), and perfluorocarbon polymer (PFCP). Tests demonstrate the sensor's ability to accurately and successively determine glucose concentrations ranging from 2.8 to 167 mM, over a 66 day period with no increase in response time, while remaining imperviousness to the effects of interference species (2.8 mM ascorbic acid, 0.3 mM uric acid, 0.3 mM p -acetaminophen). Furthermore, tests on diabetic urine samples showed an excellent correlation coefficient of 0.985 ( y =1.04 x +4.73, n =30) between sensor results and those of Glucose-Dehydrogenase clinical laboratory analyses.