Karyology of Agapanthus africanus (L.) Hoffmanns (Agapanthaceae)

The genus Agapanthus L'Heritier (Agapanthaceae) is a monocotyledonous, herbaceus and perennial plant group endemic to southern Africa. Ten species have been described for this group, in which three are considered evergreen and seven deciduous plants.  Nevertheless,  Agapanthus  L'Heritier taxonomy is considered very difficult once there are few unique characters and a high plasticity depending on circumstances of growing. This feature takes some authors to believe in the existence of only one species with innumerous varieties.The cytogenetic has been an important tool to aid the plant taxon identification, and to date, all taxa of  Agapanthus  L'Heritier studied cytologically presented 2n = 30. Although the species possess large chromosomes, few karyological studies has been explored. This work aimed to increase the cytogenetic knowledge of  Agapanthus africanus  (L.) Hoffmanns by chromosome banding techniques with DAPI / CMA 3  and Fluorescent  in situ  Hybridization (FISH). In addition, flow cytometry was used to determinate   DNA content and the percentage of AT / GC nitrogenous bases. Plants studied showed 2n = 30 chromosomes, ranging from 4.34 - 8.55 µm, with karyotype formulae (KF) = 10m + 5sm. The FISH technique revealed one 45S rDNA signal observed proximally to centromere of the chromosome 7, and one 5S rDNA signal observed proximally to centromere of chromosome 9. The 2C DNA content estimated for the species was 2C = 24.4pg, with 59% of AT and 41% of GC. Our data allowed important upgrade for biology and cytotaxonomy of  Agapanthus africanus  (L.) Hoffmanns, and will be used to future taxonomic and phylogenetics studies, as well as for the chromosome evolution of the genus. APOIO FINANCEIRO: Fapemig, Capes, CNPq