Metabolomic and genomic profiles of Streptomyces albulus with a higher ε-polylysine production through ARTP mutagenesis

Abstract Effects of atmospheric and room temperature plasma (ARTP) mutagenesis on Streptomyces albulus were investigated at cellular and molecular levels to elucidate the intracellular metabolism changes and the mechanism of e-polylysine (e-PL) yield enhancement in the mutant strain SAR 14−116 with ARTP treatment. An e-PL yield increase by 18.46 % was obtained and morphological changes of spores and mycelia were found in SAR 14−116 at a mid-logarithmic phase, compared with its original strain SAB. Kinetic characteristics of e-PL in the culture broth of SAB and SAR 14−116 were analyzed, showing obvious e-PL yield difference between these two strains. Untargeted metabolomics analysis showed there were 851 differential metabolites between these two strains. These metabolites were involved with 64 metabolic pathways through KEGG analysis. Genomic analysis showed there were 88 SNPs in SAR 14−116. The e-PL yield increase in SAR 14−116 might have a relationship with the up-regulated metabolites in the l -lysine synthesis and degradation pathways.