Characteristic antimicrobial resistance of clinical isolated strain Stenotrophomonas maltophilia CYZ via complete genome sequence.

Abstract Objective Stenotrophomonas maltophilia is an important multidrug-resistant pathogen that is associated with various of serious nosocomial infections. In our study, we investigated the traits of antimicrobial resistance of a clinical isolated strain of S. maltophilia, which was termed as CYZ from the sputum of an immunocompromised patient. Methods The whole genome sequence of S. maltophilia CYZ was investigated via the Pacbio RS II system. The functions of all the predicted genes were annotated by the COG, GO and KEGG databases. Several types of antibiotics were selected to test the antimicrobial susceptibility, and the phylogenetic tree was constructed that based on the 16S rRNA gene sequences. Results S. maltophilia CYZ has a length of 4,517,685 bp and contains 4,077 predicted genes, with an average G + C content of 66.65%. Functional genomic analysis via the annotations of the COG and GO databases revealed that the bacteria exhibited specific manners to resist the antibiotics. The annotated genes that were involved in flagella, pili or fimbriae, biofilm formation, polysaccharide, and cyclic-di-GMP may contribute to promote the ability of antimicrobial resistance. This bacterium showed sensitivity to levofloxacin, trimethoprim-sulfamethoxazole, and minocycline via antibiotics sensitivity tests. Phylogenetic relationship indicated that S. maltophilia CYZ exhibited closely relation to the strains of S. maltophilia, which were isolated from the nosocomial environment. Conclusions The current results give us a better understanding of the genetic characteristics of the antimicrobial resistance of S. maltophilia CYZ, and provide well genetic basis for further studying on the phenotype.