HBsAg, technology for preparing HBsAg through expression by virtue of recombinant saccharymyces cerevisiae, separation and purification technology for HBsAg as well as hepatitis B vaccine

The invention discloses a technology for preparing HBsAg through expression by virtue of recombinant saccharymyces cerevisiae and a separation and purification technology of the HBsAg. The separation and purification technology of the HBsAg comprises the following steps: breaking cells, namely feeding cerevisiae cells extracted after fermentation into a high pressure homogenizer and breaking the cerevisiae cells at a pressure of 15000+/-1500PSIG; heating feed liquid after breaking, namely heating the feed liquid to 50-80 DEG C and then cooling the feed liquid to 2-8 DEG C; and purifying, namely purifying the feed liquid and preparing HBsAg raw liquid. The invention also discloses a corresponding hepatitis B vaccine and HBsAg. The technology for preparing the HBsAg through expression by virtue of the recombinant saccharymyces cerevisiae has the advantages that protease can be inactivated without adding a protease inhibitor before the cells are broken, so that secondary pollution caused by the protease inhibitor is avoided; meanwhile, the yield of the raw liquid obtained through purification subsequently can be obviously increased, and quality indexes such as 'specific activity' and 'Sub-p24' of the raw liquid are greatly improved, so that the technology for preparing the HBsAg through expression by virtue of the recombinant saccharymyces cerevisiae has high economic benefit and social benefit.