Fourier transform resonance Raman spectroscopy of phytochrome.

The P r and P fr forms of phytochrome in H 2 O and D 2 O have been studied by Fourier transform resonance Raman spectroscopy with near-infrared excitation (1064 nm). It is demonstrated that this technique is a powerful method for analyzing the chromophore structures of photosensitive pigments. The high spectral quality allows discussion of vibrational assignments based on an empirical approach using previously published data obtained from model compounds