Immunochromatographic analysis method, immunochromatographic analysis device and immunochromatographic analysis kit

Provided is an immunochromatographic analysis method which does not require expert knowledge of specific devices and analysis and which does not involve complicated operations. A first target substance, which is to be determined as positive or negative, and a second target substance are individually colored by means of an immune reaction, wherein the ratio of the second target substance to the first target substance, once found, is made the standard for the determination of positive or negative. The concentration at which the strength of the coloring signals of the two target substances is the same is set as the boundary of determination for the first target substance.