Novel process for extracting golden fungus polysaccharides by step enzyme method

2007 
The invention relates to a production process for an edible and medicinal fungus polysaccharide, belonging to the biological product production technical field, which is characterized in that a water lixiviation liquid of a golden fungus is processed step by step by the means of enzymolysis via a pectinase, a cellulose, and a neutral prolease, identifying the process conditions comprising the suitable amount, enzymolysis time, temperature, pH value and the other figures, enabling the polysaccharide component inside and outside of the golden fungus solid cell to be separated fully from the non-polysaccharide big molecules such as a pectin, a cellulose, a protein and the other members, the polysaccharide component is discharged and dissolved into the water, finally, the polysaccharide is separated from the solution by the means of ethanol sedimentation. The analysis result shows compared with the traditional water lixiviation the extraction rate of the polysaccharide is increased from 7.8% to 14.3%, the protein content in the polysaccharide is lowered from 13.3% to 1.6%, and the product yield and quality are improved drastically by utilizing the stepping enzymolysis to extract the golden fungus polysaccharide. The production process for an edible and medicinal fungus polysaccharide has the advantages of efficiently extracting the golden fungus polysaccharide with high purity, completely protecting the molecular structure and the conformation of the polysaccharide, and maintaining the biological activity under the moderate production conditions.
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