Bridging scales in scattering tissues via multifocal two-photon microscopy

Imaging biological systems at subcellular resolution and across scales is essential to understanding how cells form tissues, organs, and organisms. However, existing large-scale optical techniques often require harsh tissue clearing methods that compromise the integrity of the cell membrane, cause significant morphological changes, and reduce the signal of fluorescent proteins. Here, we demonstrate multifocal two-photon microscopy that enables imaging mesoscopic scattering samples in their native tissue environment at high resolution and high speed