Antiplatelet antibodies. Evaluation of a method of indirect immunofluorescence

The sera of 55 primary autoimmune thrombocytopenic purpura patients were examined with indirect immunofluorescence method. The authors intended to verify the sensibility of an indirect method and to determine the class of detected antibody. The test has been positive in 28 of the 55 examined patients (51%). In these patients isolated or associated IgG, IgM, IgA have been detected. The method has shown a fair sensibility, a good reliability and a sure utility in those patients whose severe thrombocytopenia does not allow the execution of a direct method.