Mixed ion-pair high-performance liquid chromatography of uridine 5′-diphospho-α-d-glucuronic acid and its hydrolysis products

Abstract Although conventional ion-exchange HPLC and ion-pair reversed phase HPLC using tetrabutylammonium hydroxide allowed ready determination of uridine 5′-diphospho-α- d -glucose and its hydrolysis products, neither method was suitable for the determination of uridine 5′-diphospho-α- d -glucuronic acid (α-UDPGA). However, mixed ion-pair reversed-phase HPLC, using a combination of tetrabutylammonium hydroxide and tetraethylammonium hydroxide (1:1), has been found to offer a convenient and effective means of simultaneously determining α-UDPGA and its hydrolysis products, UDP, UMP and cUMP.