Comparison of Frozen Sections and Nonlinear Imaging for Evaluation of Mohs Surgical Margins

Rapid fluorescent labeling combined with optical sectioning and H&E-rendering algorithms can provide histological imaging of surgical specimens more rapidly than frozen sections by avoiding the need to freeze tissue and physically cut slides. Using a novel protocol that enables precise coregistration of nonlinear microscopy (NLM) and diagnostic frozen sections (FSA) produced during Mohs surgery, we present the results of a study comparing the relative accuracy of NLM and FSA.