Measurement of kidney stone formation in the rat model using micro-computed tomography
2012
Kidney stones were induced in 5 rats by treating them with 1% ethylene glycol and 1% ammonium chloride through free
drinking water for six weeks. The animals were anesthetized and imaged in vivo before the treatment at week 0, to
obtain baseline data, then at weeks 2 and 6 to monitor the kidney stone formation. Micro-CT imaging was performed
with x-ray tube voltage of 90 kV and a current of 40 mA. At week 2, kidney stone formation was observed. A micro-computed
tomography methodology of estimating the volume and hydroxyapatite-equivalent mineral content of the
kidney stone is presented. It determines the threshold CT number (390 HU) that separates the kidney stone from the
tissue. The mean volume of the stones in the 10 kidneys significantly increased from 3.81±0.72 mm3 at week 2 to
23.96±9.12 mm 3 at week 6 (p 2 =0.34). Measurement precision error was about 4%. This method allows analysis
of the kidney stone formation to be carried out in vivo, with fewer experimental animals compared with other ex vivo
methods, in which animals are sacrificed. It is precise, accurate, non-destructive, and could be used in pre-clinical
research to study the formation of kidney stones in live small animals.
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