Efficient conversion of chitin-derived carbon sources into microbial lipid by the oleaginous yeast Cutaneotrichosporon oleaginosum

Abstract Chitin represents the second most abundant biomass after lignocelluloses in the biosphere. It can be depolymerized into either N-acetylglucosamine (GlcNAc) or glucosamine (GlcN) and acetate by different degradation strategies. However, these chitin-derived carbon sources have been scarcely compared for lipid production. Here, GlcNAc was found superior to GlcN or acetate for lipid accumulation by Cutaneotrichosporon oleaginosum. The lipid accumulation potential of these carbon sources was calculated based on a small scale metabolic model of C. oleaginosum. Co-fermentation of GlcN and acetate under phosphate limitation rendered improved lipid production. GlcN and acetate were assimilated simultaneously. The highest lipid titer and yield of 10.1 g/L and 0.25 g/g, respectively, was reached when GlcNAc was used under phosphate limitation. The fatty acids composition of the lipid samples showed similarities to vegetable oils, demonstrating the suitability in biodiesel industry. This study provides profitable guidance for the design of chitin-to-lipids routes.