A NON-CONTACT MICRO THERMOCYCLING CHIP FOR POLYMERASE CHAIN REACTIONS
2003
A micro thermocycling chip has been developed, which is able to achieve high heating and cooling rates of over 16°C/s and 9.6°C/s respectively using the induction heating method. The heating element of the micro thermocycler was fabricated through electroplating on glass. SU-8 and Polydimethyl-siloxane (PDMS) are used to form the reaction chamber. DSP base servo controller was selected for close loop control the reaction chamber temperature. The thermocycler chip's properties, such as non-contact heating method, rapid cycling speed and PC control, make it suitable to integrate into clinical molecular diagnostic devices and point-of-care devices.
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