Estimation of in vitro cytotoxicity of Adhatoda vasica leaves on monkey kidney cell line

Aim: Adhatoda vasica is well known for its effectiveness in treating asthma, chronic bronchitis and other respiratory conditions. This study aims for the estimation of Nephrotoxicity of Adhatoda vasica leaves extract by in vitro cytotoxicity on Monkey Kidney cell line. Methodology: The leaves of Adhatoda vasica collected and extracted for the active principle Vasicine. In vitro cytotoxicity of the extract Vasicine on Monkey kidney cell line was performed by Microculture Tetrazolium assay. Results: The study finding infers that the extract of Adhatoda vasica leaves is safe at 10 µg, 100 µg and 250 µg doses and cytotoxic at 500 µg and 1000 µg dose concentration. Interpretation: This study infers that safe dose of Adhatoda vasica plant Extract Vasicine is upto 250ug in normal cell Cell line above which it will be toxic.