Determination of 15 perfluoroalkyl acids in chicken liver by liquid chromatography-tandem mass spectrometry.

Objective To establish a method for the determination of 15 perfluoroalkyl acids(PFAs) in chicken liver by liquid chromatography-tandem mass spectrometry(LC-MS/MS). Methods PFAs were separated on C18 column and detected by LC-ESI-MS/MS under multiple reaction monitoring(MRM) via negative-ion mode. PFAs in chicken liver were extracted with a modified NaOH digestion WAX method, investigated recoveries of 15 PFAs in LC-ESI-MS/MS determinations with different concentrations of NaOH/methanol, different temperatures and different time. Results This method showed a good linearity over the range from 0.5 μg/L to 20 μg/L for 15 PFAs with correlation coefficient r0.997. The limit of detection was 0.004～0.064 μg/kg. The limit of quantification was 0.014～0.213 μg/kg. In addition to two kinds of long carbon chain PFAs, the recoveries of other 13 PFAs were found to be in the range from 80% to 120% with relative standard deviation of 0.93%～14.5%. Conclusion This method shows highly sensitive and reproducible, and it is suitable for the detection of 15 PFAs in chicken liver.