Modulated Rotating Orthogonal Polarization Parametric Imaging, A Preliminary Study

We propose a new microscopic imaging technique in which the polarization angles of illumination light and a polarizer in front of the imaging sensor oriented orthogonally to the illumination polarization are rotated synchronously. A series of images of cervical cells was recorded under different illumination polarization angles and an algorithm was used to fit the pixel intensity variations of the images. A reconstruction method was employed to map the anisotropic properties of cervical cells in the form of a set of polarization parameters. Analysis of the images of the cervical cells and comparison with traditional methods indicate that this technique provides higher contrast and sensitivity.