Establishment of H2O2‐Induced Premature Senescence in Human Fibroblasts Concomitant with Increased Cellular Production of H2O2

Premature senescence of human fibroblasts is established after exposure to an acute sublethal concentration of H 2 O 2 . Overexpression of transforming growth factor-pl (TGF-pl) was shown to be responsible for the appearance of the biomarkers of senescence in these conditions. Other studies have shown that incubation of human fibroblasts with TGF-pl leads to overexpression of H 2 O 2 . In this work, we show an increased production of H 2 O 2 by human fibroblasts as premature senescence is established after an initial exposure to H 2 O 2 .