Expression of Vitreoscilla Hemoglobin Gene in Bacillus amyloliquefaciens and Its Effect

The Vitreoscilla hemoglobin(VHb) gene(vgb) was placed under the control of the P43 promoter by overlapping extension PCR.The vgb expression cassette was integrated into the chromosome of Bacillus amyloliquefaciens FZB42 using integrative vector pDG1730.The production of VHb confirmed by Western-Blot and CO-difference spectra analysis,promoted the growth of the strain and improved the yield of the antimicrobial lipopeptide.The maximum viable cell counts of the FZB42-VHb strain were 1.14 fold of those of the host strain.Under the same conditions,the yeild of the antimicrobial lipopeptide fengycin and surfactin were 1.74-,3.14-fold of those of the host strain,respectively.