Construction of a Tributyrin Calibration Curve using High Performance Liquid Chromatography

Most of the explored research works on Triglycerides or any of the Triglycerides families was based on the profiling identification than a quantification. To quantify them, a calibration curve needs to be constructed at a certain range of concentration. In this study, the calibration curve for the shortest chain of Triglycerides namely Tributyrin was accomplished using high performance liquid ultraviolet detector with two variables parameters. The composition of mobile phase for tributyrin was found optimum using 50:50 acetonitrile: acetone while the optimum flowrate for tributyrin was 0.55 mL/min using a 20 μL injection, constant condition at 35°C and 215 nm wavelength. The calibration curve of standard tributyrin was found linear from 0.0025-0.0375 mM with the correlation coefficient (R2) of 0.986. The developed calibration curve could serve as a future platform for the quantification of TB in real samples with various matrixes such as food and serum.