Building the long, periodic ssDNA nanotemplate from tumor marker recognition for nanodevice

The programmable self-assembly of molecular nanostructures plays a crucial role in the development of nanodevices for molecule recognition. A self-assembly, periodic ssDNA nanotemplate based on aptameric recognition of tumor marker, PDGF was in situ constructed on the silicon-based substrate. The long, periodic ssDNA nanotemplate was further elongated up to 454 nm through rolling circle amplification at room temperature. The process of self-growing DNA nanotemplate based on aptameric recognition was directly visualized through nanoprobing technology.