Optimization of biomethane production in mono-cardboard digestion: key parameters influence, batch test kinetic evaluation and DOM indicators variation

Mono-cardboard waste digestion in batch tests associated with different impact factor were investigated. The maximum methane generation was 394 mL/gVSadd with a best F/M of 0.5 at mesophilic condition. The highest methane content reached to 75% while in the shocking water bath feeding with an average particle of 1-3 mm. The hydrolysis and methanogenesis was significantly different between static and shock state fermentation, especially at particle size over than 3 mm. The modified Gompertz model (R2>0.98) and modified Aiba model (R2>0.88) were the most appropriate model for methane generation among the six kinds models. At different TS，the variation of dissolved organic matters (DOM) reflects the metabolic rate of microbial community. The soluble microbial product-(SMP) like and protein-like components half split by excitation–emission matrix–parallel factor (EEM–PARAFAC) were significantly negative corresponded to bio-methane production. Moreover, a rapid loss of methanogenesis was obtained with high org...