Влияние ß-адреноблокаторов на рост кардиомиоцитов в культуре ткани сердца

The method of organotypical cell culture was used. The long-term cell culture of cardiac embryonic tissue of 10-12-days old chicken was investigated. The effects of s 1-adrenergic blockers (atenolol, metoprolol) and noradrenaline on the growth of explantat cardiac tissue were measured quantitatively. It was found that s 1-adrenergic bloker atenolol in 10 -6 M and 10 -4 M concentration stimulated growth of cardiac tissue on 23% and 40% correspondently. Atenolol in 10 -8 M concentration, contrary, depressed growth of explantat. Metoprolol (10 -12 10 -4 M) inhibited the growth of explantat cardiac tissue. Noradrenaline (10 -12 M) stimulated growth of explantat cardiac tissue. However the joint action of noradrenaline (10 -12 M) and metoprolol or atenolol did not influence on growth explantat cardiac tissue. These results suggest that cardiac tissue stimulating effect of noradrenaline mediated through activation of s 1-adrenergic receptors.