Detection of DNA Adducts by 32P-Postlabeling and Multifraction Contact-Transfer Thin-Layer Chromatography

Abstract Detection of DNA Adducts by 32 P-Postlabeling and Multifraction Contact-Transfer Thin-Layer Chromatography. Laws, G. M., Adams, S. P., Nichols, W. W., and Selden, J. R. (1994). Fundam. Appl. Toxicol. 23, 308-312 32 P-Postlabeling is a sensitive method for detecting DNA adducts. Large bulky adducts, particularly from polycyclic aromatic compounds, are readily detected using this technique. Detection of small modifications, such as methylations, has often required specific additional enrichment procedures prior to 32 P-postlabeling. We report the use of a single analytical procedure that can detect DNA adducts of a wide range of sizes and hydrophobicities (exemplified by adducts procedured with methyl methanesulfonate, diepoxybutane, styrene oxide, or benzo[ a ]-pyrene). This 32 P-postlabeling/thin-layer chromatography procedure is particularly useful when examining the potential of novel compounds or their metabolites to form DNA adducts.