Active components and mechanism of Tibetan medicine Gentiana straminea against cytokine storm

Objective: To analyze the main active components and possible mechanism of Mahuaqinjiao (Gentiana straminea) in the preventive treatment of cytokine storm by network pharmacology Methods: The therapeutic targets for cytokine storms and its active ingredients of G straminea were searched and collected from the database of TCMSP and GeneCards;The regulatory network map of active component cytokine storm targets of G straminea was constructed by using Cytoscape 3 6 1;The network map of protein-protein interaction was constructed to screen the core target, and the gene ontology (GO) function enrichment analysis and KEGG pathway enrichment analysis of the core protein were carried out by using the online database of DAVID Results: After screening, 14 active components and 31 effective targets for prevention and treatment of cytokine storm were obtained, mainly including IL6, STAT3, Jun, AKT1, EGFR, MMP9, VEGFA, IL-1β, IL-10, and other core targets Go analysis showed that G straminea mainly involved in the regulation of apoptosis, gene expression, cytokine activity, positive regulation of nitric oxide biosynthesis, growth factor activity, immune response and other processes KEGG pathway analysis showed that the main pathways related to cytokine storm were T-cell receptor signaling pathway, JAK STAT signaling pathway, HIF-1 signaling pathway, tumor necrosis factor signaling pathway, Toll like receptor signaling pathway, PI3K Akt signaling pathway and other core target pathways Conclusion: Based on the data mining of network pharmacology, the potential active components and possible mechanism targets of G straminea against cytokine storm were predicted preliminarily, in order to provide reference for the application of G straminea in the prevention and control of cytokine storm, and provide ideas for the development of new uses of G straminea