EFEK PAPARAN RADIASI SINAR-X PADA PERUBAHAN MOTILITAS DAN MORFOLOGI SPERMA TIKUS STRAIN WISTAR

Material and Methods: This research is an in vivo experimental research using Winstar Rats . in this research, the treatment group was exposed to various X-ray radiation : 50 mGy, 100 mGy, and 2000 mGy compared to one control group which was untreated group. Each group was contain of 6 Winstar Rats. Radiation exposure was calibrated using RTI Piranha dosimetry. Rats were exposed to X-Ray radiation until reach the desire radiation doses. After 30 days, rats was sacrificed and left orcidectomy was performed. Sperm motility was assesed using direct observation and Hematoxylin Eosin (HE) stain was used to assesed viability using light microscope. One Way ANOVA was used to compare motility and viability of rats spermatozoa in treatment group compared to control group with a significant value of p <0.05. The analysis was continued with a post hoc test, to determine the differences in each group