Expression and localization of leptin and its receptor in mammary gland of dairy cow

The protein expression and localization of leptin and its long form receptor(OB-Rb) were detected by western blottig methord and a confocal laser scanning microscope.The results showed that leptin protein level was higher in virgin,the lowest in lactation.Then OB-Rb was higher in virgin and pregnancy,lower in lactation,and recovered to the original level in involution.