Improved production of Oroxylum indicum (L.) Kurz by altering the salts of the medium

The present study was conducted to test the effects of KNO3, KH2PO4, and CaCl2 on shoot multiplication, root proliferation, and accumulation of phytochemicals in in vitro cultures of Oroxylum indicum. The results indicate that modifying the MS salt formulation in relation to particular inorganic nutrients highly affected shoot multiplication, root proliferation, and accumulation of flavonoids in in vitro cultures. A concentration of 0.60 g L−1 CaCl2 resulted in the highest frequency of shoot regeneration (5.6 shoots per explant). A concentration of 0.40 g L−1 CaCl2 resulted in the highest frequency of root regeneration (7.8 roots per shoot). Modifications of the concentrations of inorganic salts were also found to be advantageous for production media for both multiple shoots and shoot-derived root in vitro cultures. Multiple shoots generated on shoot induction medium with a concentration of 0.60 g L−1 CaCl2 and roots generated on root induction medium with a concentration of 1.5 g L−1 KNO3 yielded about a five times higher flavonoid level than cultures generated on control medium respectively.