Interactive effects of volatile anesthetics, verapamil, and ryanodine on contractility and calcium homeostasis of isolated pregnant rat myometrium

OBJECTIVE: Both volatile anesthetics and Ca 2+ antagonists decrease uterine contractility. The interactive effects of anesthetics, verapamil, and ryanodine on myometrial muscular activity and on intracellular Ca 2+ availability were examined. STUDY DESIGN: The effects of minimum alveolar concentrations of 0.5, 1.0, and 2.0 of halothane, enflurane, and isoflurane on electrically stimulated isometric mechanical activity of isolated longitudinal myometrium strips from 45 pregnant rats (15 to 21 days), superfused with Krebs' solution with or without 5.0 mmol/L Ca 2+ , 10 −7 and 10 −6 mol/L verapamil, and 10 −6 mol/L ryanodine, were compared. Analysis was performed by analysis of variance and Duncan test. RESULTS: All three anesthetics and verapamil produced dose-dependent depression of contractility. Increasing the Ca 2+ concentration from 2.5 to 5.0 mmol/L in the superfusate partly reversed the depression. An additive effect of verapamil and anesthetics, especially enflurane, on contractile force was observed. Ryanodine had no effect on contractility, but it could partly counteract the depressant effect of anesthetics. CONCLUSION: Clinically used concentrations of volatile anesthetics modify Ca 2+ availability and depress uterine contractility. General anesthesia, especially by enflurane, in patients being treated with calcium antagonists may represent a higher risk.