Sodium Butyrate Inhibits the Inflammation of Lipopolysaccharide-Induced Acute Lung Injury in Mice by Regulating the Toll-Like Receptor 4/Nuclear Factor κB Signaling Pathway

Bacterial pneumonia is a common disease in dairy herds worldwide, which brings great economic losses to farmers. Sodium butyrate (SB), an inhibitor of histone deacetylase, plays an important role in limiting inflammation. The purpose of this study was to investigate the protective effects of SB on LPS-induced acute lung injury (ALI) in mice and explore the potential mechanism of SB protection. Thirty ICR mice were randomly divided into three groups (n=10): a PBS intratracheal instillation group, an LPS intratracheal instillation group, and an SB gavage group (SB was given 1 h before the LPS stimulation). After 12h, samples of the blood and lung tissue were collected from the mice for experimental analysis. The results showed that the concentration of inflammatory cytokines (IL1β and TNF-α); myeloperoxidase (MPO) activity in the lung tissue and blood; the protein abundance of Toll-like receptor 4 (TLR4), nuclear factor kappa B (P65), phosphorylated P65 (p-P65), inhibitor κBα (IκBα) and phosphorylated IκBα ...