Syphilis cardiolipin and specific antibody IgG (Immunoglobulin G) immunoblotting kit and preparation method thereof

The invention discloses a syphilis cardiolipin and specific antibody IgG (Immunoglobulin G) immunoblotting kit and a preparation method thereof, relating to a kit. The kit is provided with a vector plate, a nitrocellulose membrane, a syphilis specific IgG antibody and cardiolipin IgG antibody detecting line and a contrast line, wherein the detecting line and the contrast line are arranged on the nitrocellulose membrane in sequence; a syphilis specific recombination antigen and a cardiolipin antigen are coated at the syphilis specific IgG antibody and cardiolipin IgG antibody detecting line, and a human IgG antibody is coated at the contrast line; and a horse radish peroxidase is marked with an antihuman gamma chain antibody. The preparation method comprises the following steps of: preparing the syphilis specific recombination antigen firstly and then preparing the cardiolipin antigen; then sampling the nitrocellulose membrane; preparing the antihuman IgG specific fragment gamma chain monoclonal antibody; and after marking the horse radish peroxidase with the antihuman IgG specific fragment gamma chain monoclonal antibody, preparing the immunoblotting kit. The syphilis cardiolipin and specific antibody IgG immunoblotting kit can be used for the detection of a syphilis specific IgG antibody and a cardiolipin IgG antibody in specimens, such as whole blood, serum, blood plasma, cerebrospinal fluid, and the like.