Study on distribution of neural stem cells in the brain of Alzheimer disease transgenic mice through caudal vein transplantation

Objective To observe whether neural stem cells(NSCs)can successfully permeate into the brain through the blood-brain barrier(BBB)of Alzheimer disease(AD)transgenic mice and explore the methods of distribution and migration.Methods NSCs were isolated from 12-day-old fetal mice,cultured, labeled with enhanced green fluorescent protein(eGFP)and then transplanted into 10 AD transgenic mice and normal mice as controls through caudal vein.The mice were killed 48 h,1 w,2 w,and 4 w after transplantation respectively.The brains of the mice were made into continual frozen sections,the distribution and migration of the eGFP-labeled NSCs were studied under fluorescence microscope.Results At different time points after transplantation the eGFP-labeled NSCs were diffusely distributed in the brain:distributed around the blood vessels in the first 48h,and then migrated gradually towards the hippoeampus and cortex until 4 weeks later.There were no obvious abnormal complications occurring after transplantation. Conclusion NSCs can successfully permeate into the brain through the BBB of AD transgenic mice,and migrate into the brain parenchyma gradually.