Analysis the result of irregular antibody test in Rh(D) negative donors

Objective To analyze the result of irregular antibody test in Rh(D) negatie donors,discuss in the necessity of irregular antibody test in Rh(D) negative confirmation experiment among the blood testing with Rh(D) negative donors.Methods Rh(D) negative confirmation experiment antibody screening and antibody Identification test using indirect antiglobulin test(IAT),The Rh phenotype were determined by saline method.Results among 1205 samples of Rh(D) negative donors,20 samples antibody screening test is positive.And after antibody Identification test,Among them 10 samples were anti-D,6 samples were anti-M,2 samples were Nonspecific antibody,1 sample was anti-Lea,1 sample was cold antibody.Conclusion The positive rate of Antibody screening in Rh(D) negative donors is 1.66%.it is greater than Previous reports of the normal population positive antibody screening.the higher positive rate of Antibody screening has Important clinical significance in blood supply.therefore Rh(D) negative donors more should do irregular antibody test.